Employment of Near Full-Length Ribosome Gene TA-Cloning and Primer-Blast to Detect Multiple Species in a Natural Complex Microbial Community Using Species-Specific Primers Designed with Their Genome Sequences

Huimin Zhang, Hongkui He, Xiujuan Yu, Zhaohui Xu, Zhizhou Zhang

Research output: Contribution to journalArticlepeer-review

Original languageEnglish
Pages (from-to)729-737
Number of pages9
JournalMolecular Biotechnology
Volume58
Issue number11
DOIs
StatePublished - Nov 1 2016

ASJC Scopus Subject Areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

Keywords

  • Full-length rRNA
  • Genome sequence
  • Primer-Blast
  • qPCR
  • Species-specific primer

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